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KMID : 1098420070150050315
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 5 p.315 ~ p.318
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Rapid Propagation through Tissue Culture of Cudrania tricuspidata, Medicinal Plant
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Lee Cheul-Ho
Kim Kyu-Siek
Choi Myung-Suk
Min Ji-Yun
Jung Ha-Na
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Abstract
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An effective rapid propagation method was established through in vitro cultures of the medicinal plant, Cudrania tricuspidata. In vitro plantlets were obtained from in vitro germinated seeds. The various levels of cytokinins (BAP, Kinetin and TDZ) were tested on multiple shoot formation from plantlets. BAP (1.0 mg/l) treatment induced highest number of multiple shoots. Single shoot cultures gave higher initial shoot numbers than 5 shoots per culture. Among the various culture media, the shoot elongation was optimal on 2 MS basal medium without growth regulators. The IAA (2.0 mg/l) treatment induced highest number of roots. IBA (2.0 mg/l) treatment more promoted in vitro root growth than other concentrations. Rooted shoots were transferred directly to small pots with an artificial soil and successfully acclimatized.
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KEYWORD
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Cudrania tricuspidata, cytokinin, multiplication, in vitro rooting
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